Technology without application is only an exercise...
Here is something done by/with OnePot!
Application Fields
Soft Biotech
Pharma
Biocatalysis
OnePot Gain
46% Time Saved
1 Device for Incubation, Sterilization and Induction
Translated in 7 WDs
A classical protocol for producing enzymes (CV-wTa) by bacterial cell culture (E. Coli) has been translated on OnePot. The process consists of the following steps: LB medium preparation and sterilisation, E. Coli pre-culture, E. Coli central culture, induction of enzyme expression by IPTG solution, and harvesting. Cell culture yield has been measured by the cellular density (ODD) ratio. The enzyme expression was evaluated by SDS-page analysis.
E.Coli cell culture and CV-wTa enzyme production
Synthesis of Monastrol
Application Fields
Organic Synthesis
Pharma
OnePot Gain
x500 scaleup
Reaction efficency comparable to MW
Translated in 5 WDs
Monastrol has been synthesised by Biginelli reaction following an MW-assisted protocol previously reported by Kappe et al. The multicomponent reaction between thiourea, 3-OH-benzaldehyde, and ethylacetoacetate is catalysed by Yt(OTf)3 under MW heating at 120°C for 30 min. Compared to classical protocols, MW heating is an instant on-off internal heating system that reduces reaction time and byproducts. Matrix-in-batch technology, implemented by OnePot, is comparable to MW in terms of efficiency on a bigger scale.
N-Boc protection of amino acids
Boc protection of amines is a classical procedure of organic chemistry to mask amine group
reactivity. The classical protocol, reacting the amino acid tyrosine in a basic solution by
NaOH at room temperature, followed by HCl neutralization and liquid/liquid extraction, has
been fully reproduced in an automated way.
Application Fields
Organic Synthesis
Pharma
Biomaterials
Polymer Chemistry
OnePot Gain
99% Time Saved
99% Energy Saved
Scaled in 5 WDs
Scale-Up of Zein Nanoparticles Production
Application Fields
Cosmetics
Food & Nutrition
Pharma
Biomaterials
OnePot Gain
99% Time Saved
99% Energy Saved
Scaled in 5 WDs
Zein is a prolamin derived from corn that was proposed for several pharmaceutical and food applications due to its safety profile and low
immunogenicity. Zein-based nanoparticles were prepared by means of the nanoprecipitation technique. In detail, the traditional method was based
on the use of an initial volume of 8 ml of solvent (containing 10 mg of protein), while the OnePot®-assisted process was performed on 320 ml of
formulation (corresponding to ~40 cumulative traditional batches, 400 mg of protein).
Synthesis of PVP K-90 polymer
Povidone (polivnyl pyrrolidone, PVP) polimer has been synthetized following a procedure for the radical polymerization of 1-vinyl-
2-pyrrolidone (VP) activated by 2 2'-azobis(2-methyl)propionitrile (AzBn) as radical initiator. Reaction was controlled by relative
viscosity measure of a 10% water solution of product. Depending on reaction time and temperature it is possible to obtain
different degree of polymerization for different type of PVP, characterized by different K-values, related to relative viscosity in
water. European Pharmacopeia admits PVP with K-value ranging between K-12 and K-90.
Application Fields
Cosmetics
Food & Nutrition
Pharma
Material Chemistry
Polymer Chemistry
OnePot Gain
40% Time Saved
45% Energy Saved
Controlled Polymerization degree
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Is there any hidden cost?There will be no hidden costs whatsoever. You just have to consider potential delivery charges (if you choose the month-to-month subscription) and extra fees if you want more accessories (e.g. an extra vessel). We at Katakem consider transparency to be very important because it speeds up things for you as well as for us. Therefore we do our best to make sure everything is as straightforward and clear as possible. Please contact us if you are unsure or have any questions.
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Can I run a test before subscribing?Sure! We'd be glad to welcome you in our lab to test OnePot. Alternatively, you can run a 3-month trial in your lab.
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Do you provide an installation service?Yes, it is included in the annual subscription, whereas it costs 987€ when you choose the month-to-month subscription (shipping included).
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What kind of OnePot do I get when I subscribe?"Mint condition" means that the OnePot you get will look and feel as good as new, even though it's been in labs before. This helps us get the most use out of every robot we produce, making chemistry more sustainable for everyone. All OnePots get regular maintenance, their connectivity features are frequently updated and of course professional cleaning inside and out in between members to keep them in prime condition.
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CommitmentTo end an existing contract, contact us at least 20 days before the next billing period. Once you have notified us via email or a phone call, we’ll help you book a date to return your OnePot. You can of course keep using your OnePot until the return date.
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Maintenance & ServiceWith OnePot's connected features, there's no need to keep track of your maintenance schedule. When it's time for maintenance, we will contact you to schedule a day and time that's convenient for you. Then we'll come to your lab, fresh OnePot up, and handle it back to you. In case anything brakes up, call us and in 48 hours we will be in your lab to fix it.
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Can I change my subscription from monthly to annual?Yes, you can do it anytime. Please, talk to your account manager.
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What's a swap?After your OnePot has been in the lab for a while, we’ll contact you to swap it out for a fresher version. We’ll try to minimise the hassle for you, while ensuring you get a new OnePot. More details about swaps are on the way!
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What payment methods do you accept?As for now, we only accept bank transfer. We are working on offering different options in the near future.
Common Questions
Sooner or later, chemists will find solutions to all the problems that affect our Planet and humanity.
Our mission is to provide chemists with the best tech ever to let them achieve this goal sooner rather than later.
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