Technology without application is only an exercise...
Here is something done by/with OnePot!
Application Fields
Soft Biotech
Pharma
Biocatalysis
OnePot Gain
46% Time Saved
1 Device for Incubation, Sterilization and Induction
Translated in 7 WDs
A classical protocol for producing enzymes (CV-wTa) by bacterial cell culture (E. Coli) has been translated on OnePot. The process consists of the following steps: LB medium preparation and sterilisation, E. Coli pre-culture, E. Coli central culture, induction of enzyme expression by IPTG solution, and harvesting. Cell culture yield has been measured by the cellular density (ODD) ratio. The enzyme expression was evaluated by SDS-page analysis.
E.Coli cell culture and CV-wTa enzyme production
Synthesis of Monastrol
Application Fields
Organic Synthesis
Pharma
OnePot Gain
x500 scaleup
Reaction efficency comparable to MW
Translated in 5 WDs
Monastrol has been synthesised by Biginelli reaction following an MW-assisted protocol previously reported by Kappe et al. The multicomponent reaction between thiourea, 3-OH-benzaldehyde, and ethylacetoacetate is catalysed by Yt(OTf)3 under MW heating at 120°C for 30 min. Compared to classical protocols, MW heating is an instant on-off internal heating system that reduces reaction time and byproducts. Matrix-in-batch technology, implemented by OnePot, is comparable to MW in terms of efficiency on a bigger scale.
N-Boc protection of amino acids
Boc protection of amines is a classical procedure of organic chemistry to mask amine group
reactivity. The classical protocol, reacting the amino acid tyrosine in a basic solution by
NaOH at room temperature, followed by HCl neutralization and liquid/liquid extraction, has
been fully reproduced in an automated way.
Application Fields
Organic Synthesis
Pharma
Biomaterials
Polymer Chemistry
OnePot Gain
99% Time Saved
99% Energy Saved
Scaled in 5 WDs
Scale-Up of Zein Nanoparticles Production
Application Fields
Cosmetics
Food & Nutrition
Pharma
Biomaterials
OnePot Gain
99% Time Saved
99% Energy Saved
Scaled in 5 WDs
Zein is a prolamin derived from corn that was proposed for several pharmaceutical and food applications due to its safety profile and low
immunogenicity. Zein-based nanoparticles were prepared by means of the nanoprecipitation technique. In detail, the traditional method was based
on the use of an initial volume of 8 ml of solvent (containing 10 mg of protein), while the OnePot®-assisted process was performed on 320 ml of
formulation (corresponding to ~40 cumulative traditional batches, 400 mg of protein).
Synthesis of PVP K-90 polymer
Povidone (polivnyl pyrrolidone, PVP) polimer has been synthetized following a procedure for the radical polymerization of 1-vinyl-
2-pyrrolidone (VP) activated by 2 2'-azobis(2-methyl)propionitrile (AzBn) as radical initiator. Reaction was controlled by relative
viscosity measure of a 10% water solution of product. Depending on reaction time and temperature it is possible to obtain
different degree of polymerization for different type of PVP, characterized by different K-values, related to relative viscosity in
water. European Pharmacopeia admits PVP with K-value ranging between K-12 and K-90.
Application Fields
Cosmetics
Food & Nutrition
Pharma
Material Chemistry
Polymer Chemistry
OnePot Gain
40% Time Saved
45% Energy Saved
Controlled Polymerization degree
Common Questions
Sooner or later, chemists will find solutions to all the problems that affect our Planet and humanity.
Our mission is to provide chemists with the best tech ever to let them achieve this goal sooner rather than later.
PRIVACY POLICY
TERMS OF USE
NEWSLETTER
Copyright B4CHEM SRL 2023. All Rights Reserved.
Powered by
