E.Coli cell culture and CV-wTa enzyme production

A classical protocol for producing enzymes (CV-wTa) by bacterial cell culture (E. Coli) has been translated on OnePot. The process consists of the following steps: LB medium preparation and sterilisation, E. Coli pre-culture, E. Coli central culture, induction of enzyme expression by IPTG solution, and harvesting. Cell culture yield has been measured by the cellular density (ODD) ratio. The enzyme expression was evaluated by SDS-page analysis.